Experimental design, preprocessing, normalization and differential expression analysis of small RNA sequencing experiments

Table 2 Comparison of sRNA normalization methods^a

Method	Computational complexity	Control required	Units normalized
Total count scaling	Low	No	Reads
Quantile-based scaling	Medium	No	Reads
TMM	High	Yes	Reads
Linear regression	High	Yes	Reads
Nonlinear regression	Very high	Yes	Genomic windows